UW-Madison School of Pharmacy

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Warren Heideman, Ph.D.

Professor
Associate Dean for Research

 

 

 


Cell Cycle Regulation. We are working to understand how yeast regulate the cell cycle in response to external signals. Cell cycle regulation is an important process in that improper cell cycle regulation can lead to cancer. It is also an important in biology because organized regulation of proliferative growth is a fundamental requirement for an organism, and absolutely required for the proper development of muticellular structures. More specifically, we are studying the connections between external signals and the conserved cyclin/cyclin dependent kinase (CDK) pathway that is thought to regulate progress from the G1 phase of the cell cycle into S phase. Yeast have proteins that are homologs to the products of the human p21ras oncogenes that are associated with many cancers. In yeast, the mitogen signal that regulates Ras is glucose. We are interested in how signals that control proliferation in yeast are coupled through Ras activity to the machinery of the cell cycle. Recently, we have identified Cln3, a G1 cyclin, as a major target for the Ras/cAMP pathway in yeast. We are also studying the transcriptional regulation of a number of yeast cell cycle genes to better understand how signals that produce proliferation in yeast control movement from G1 to S phase.

Developmental Toxicology. We are interested in studying the molecular mechanisms by which TCDD (dioxin) disrupts the normal developmental programing of fish. TCDD is a widespread environmental toxicant that accumulates in the food chain. TCDD activates a transcription factor, the arylhydrocarbon receptor (AhR) that in turn alters the pattern of gene expression. While it is assumed that alterations in gene expression lead to the toxic effects, it is not known what genes are critical in this response. Early life stage fish are more sensitive to TCDD than any other known organism. Because of their well known utility in genetic and developmental studies, we have established the zebrafish as a model system for studying this toxicity. In an attempt to identify genes that play a role in TCDD toxicity, we have initiated a selection for zebrafish mutants that are resistant to TCDD lethality.

Background: Warren received B.A. degrees in Zoology and Chemistry (1977), and a Ph.D. degree (1983) in Pharmacology from the University of Washington. He did postdoctoral work in the Department of Microbiology and Immunology at the University of California-Berkeley, and in the Department of Pharmacology at the University of California-San Francisco before joining the U.W. School of Pharmacy faculty in 1988. He holds faculty appointments in Biomolecular Chemistry, Molecular & Cellular Pharmacology, and Environmental Toxicology. He is also a Leukemia Society Fellow. His research interests center around signal transduction across biological membranes.

Professional Interests: Signal transduction across biological membranes

Education:

  • B.A. 1977 Zoology & Chemistry - University of Washington
  • Ph.D. 1983 Pharmacology - University of Washington
  • Postdoctoral Microbiology/Immun. - University of California-Berkeley
  • Postdoctoral Pharmacology - University of California-San Francisco
Highlighted Publications:
  • SA Carney, RE Peterson and W Heideman. TCDD Activation of the AHR/ARNT Pathway Causes Toxicity Through a CYP1A-Independent Mechanism in Zebrafish. Molecular Pharmacology 2004 (in press)
  • AL Prasch, EA Andreasen, RE Peterson, and W Heideman. Interactions between TCDD and hypoxia signalling pathways in zebrafish: Hypoxia decreases responses to TCDD in zebrafish embryos. Toxicol. Sci. [Epub ahead of print-2003 Dec 22] (In Press 2004)
  • AJ Hill, SM Bello, AL Prasch, RE Peterson, and W Heideman. Water permeability and TCDD-induced edema in early life stages of zebrafish. Toxicol. Sci [Epub ahead of print-2004 Jan 12] (In Press 2004).
  • TL Laabs, DD Markwardt, LL Newcomb, D Stillman, and W Heideman. ACE2 is required for daughter cell-specific G1 delay in Saccharomyces cerevisiae. PNAS 100(18):10275-80 (2003). (See note J Cell Biol 162: 961)
  • LL Newcomb, JA Diderich, MG Slattery, and W Heideman. Glucose regulation of S. cerevisiae cell cycle genes. Eukaryotic Cell 2:143-149 (2003).
  • EA Andreasen, RL Tanguay, RE Peterson, and W Heideman. Identification of a critical amino acid in the aryl hydrocarbon receptor. J Biol Chem 277:13210-13218 (2002)
  • LL Newcomb, DD Hall, and W Heideman. AZF1 is a glucose dependent positive regulator of CLN3 transcription in Saccharomyces cerevisiae. Mol Cell Biol 22:1607-1614 (2002)